کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2021503 | 1069249 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli](/preview/png/2021503.png)
چکیده انگلیسی
Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent indoor airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 was cloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This allergen was purified by immobilized Ni2+-affinity chromatography. The purified Pen b 26 was characterized by immunological, biochemical and biophysical methods. C-His6 tagged Pen b 26 produced several fold greater yield than N-His6 tagged Pen b 26. The affinity of C-His6 tagged Pen b 26 for the specific antibody was also 2.75 times higher than N-His6 tagged Pen b 26.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 65, Issue 1, May 2009, Pages 8–14
Journal: Protein Expression and Purification - Volume 65, Issue 1, May 2009, Pages 8–14
نویسندگان
M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay,