کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021725 1069259 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis
چکیده انگلیسی

Mytilus californianus foot protein three (Mcfp-3) was successfully expressed in the yeast, Kluyveromyces lactis. The first nine amino acids (YPYDVPDYA) from the human-influenza-virus hemagglutinin (HA) protein were fused to the amino terminus of Mcfp-3 (HA–Mcfp-3) to facilitate identification and purification. HA–Mcfp-3 was purified to a concentration of 1 mg/L using HA affinity chromatography. The recovered polypeptide was resolved by SDS–PAGE and migrated primarily at 36 kDa, an increase of approximately 29 kDa over the calculated molecular weight of a HA–Mcfp-3 monomer. Significantly, release of Mcfp-3 by enterokinase treatment coincided with the formation of high molecular weight complexes. It is noteworthy that the complexes mimicked the previously reported insolubility of Mcfps found in vivo to denaturing and reducing conditions. These data demonstrate the successful expression of Mcfp-3 in K. lactis and show an intrinsic ability of Mcfp-3 to self-assemble into stable, higher molecular weight forms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 57, Issue 1, January 2008, Pages 57–62
نویسندگان
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