کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2021748 | 1069261 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of homo- and heteropolymers of plant ferritin subunits using an in vitro protein expression system
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Ferritin is a class of iron storage protein composed of 24 subunits. Although many studies on gene expression analyses of plant ferritin have been conducted, the functions and oligomeric assembly of plant ferritin subunits are still largely unknown. In order to characterize the ability to form multimeric protein shells and determine the iron incorporating activity, we produced ferritin homo- and heteropolymers by expressing four cDNAs of ferritin subunits from soybean, sfer1, sfer2, sfer3, and sfer4, using an in vitro protein expression system. Using SDS-PAGE analysis followed by Prussian blue stain, homopolymers of SFER1, SFER2, and SFER3, and heteropolymers of SFER1/SFER2 and SFER1/SFER3 were detected as assembled polymers with iron incorporating activity, whereas only a small amount of SFER4 related homo- and heteropolymer was detected, suggesting that the SFER4 was not competent for oligomeric assembly, unlike every other ferritin. We conclude that certain combinations of plant ferritin subunits can form heteropolymers and that their iron incorporating activities depend on the formation of multimeric protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 56, Issue 2, December 2007, Pages 237-246
Journal: Protein Expression and Purification - Volume 56, Issue 2, December 2007, Pages 237-246
نویسندگان
Taro Masuda, Fumiyuki Goto, Toshihiro Yoshihara, Toru Ezure, Takashi Suzuki, Shinichiro Kobayashi, Masamitsu Shikata, Shigeru Utsumi,