کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021908 1069267 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mouse Aurora A: Expression in Escherichia coli and purification
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mouse Aurora A: Expression in Escherichia coli and purification
چکیده انگلیسی

Aurora kinases have recently become some of the most intensely pursued oncology targets for the design of small-molecule inhibitors. Most of the active Aurora-A protein variants are currently being expressed from baculoviruses in insect cells, while catalytically impaired proteins can also be generated in and purified from Escherichia coli. In this study we present a method of expressing large quantities of active mouse Aurora-A kinase domain as an N-terminal glutathione-S-transferase fusion protein in bacteria and outline a simple purification method that produces greater than 99% pure protein samples suitable for enzymatic assays and X-ray crystallography. The methods described in this report simplify mouse Aurora-A expression and purification, and may aid in the production of other difficult kinases in prokaryotes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 54, Issue 1, 1 July 2007, Pages 139–146
نویسندگان
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