کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2022038 1069276 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein expression and preliminary crystallographic analysis of amino-terminal fragment of urokinase-type plasminogen activator
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Protein expression and preliminary crystallographic analysis of amino-terminal fragment of urokinase-type plasminogen activator
چکیده انگلیسی
The amino-terminal fragment (ATF, Ser1-Glu143) of urokinase-type plasminogen activator (uPA) is responsible for some important functions of uPA, such as receptor binding and chemotactic activity. To dissect the function and structure-activity relationship of ATF, recombinant human ATF was expressed in Pichia pastoris system at a yield of about 30 mg/L. The recombinant ATF was captured by a cation exchange column, further purified up to 99% purity by a gel filtration column, and characterized in terms of its receptor binding capability. The purified ATF was then crystallized by the method of sitting-drop vapor diffusion with magnesium sulfate as the precipitating agent at 298 K. The crystals belong to space group P1 with unit cell dimensions of a = 47.5 Å, b = 64.7 Å, c = 65.4 Å, α = 71.6°, β = 92.1°, γ = 84.0°.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 49, Issue 1, September 2006, Pages 71-77
نویسندگان
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