کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2022113 | 1069278 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Bacterial production of biologically active canine interleukin-1β by seamless SUMO tagging and removal Bacterial production of biologically active canine interleukin-1β by seamless SUMO tagging and removal](/preview/png/2022113.png)
Interleukin 1β (IL-1β) is a potent stimulator of extracellular matrix degradation in models of osteoarthritis (OA). In contrast to bovine explant models which effectively respond to recombinant human IL-1β, canine models are relatively refractory to human IL-1β stimulation. Canine IL-1β cDNA was cloned in order to produce a fully potent species matched preparation of IL-1β for use specifically in canine models of OA. Established methods for the production of various orthologous IL-1β proteins from different species are problematic due to the exquisite sensitivity of the mature IL-1β product to N-terminal variations and the intrinsic technical challenges associated with producing an unmodified product. We have applied a seamless method of SUMO tagging and removal in order to produce a homogeneous unmodified preparation of canine IL-1β from Escherichia coli which was found to be a potent inducer of aggrecanase activity in isolated canine articular chondroctyes. This method combines highly efficient aspects of seamless plasmid engineering, protein purification, and precise tag removal.
Journal: Protein Expression and Purification - Volume 50, Issue 1, November 2006, Pages 102–110