A hybrid of cells and pancreatic islets toward a new bioartificial pancreas

• DNA can be immobilized onto cell by DNA–PEG-lipid to induce cell–cell attachment.
• Cell surface engineering with DNA–PEG-lipid can develop 3D cell hybrid.
• 3D hybrid of pancreatic islet and cells can be potential for type 1 diabetes cure.

Cell surface engineering using single-stranded DNA–poly(ethylene glycol)-conjugated phospholipid (ssDNA–PEG-lipid) is useful for inducing cell–cell attachment two and three dimensionally. In this review, we summarize our recent techniques for cell surface engineering and their applications to islet transplantation. Because any DNA sequence can be immobilized onto the cell surface by hydrophobic interactions between ssDNA–PEG-lipid and the cellular membrane without impairing cell function, a cell–cell hybrid can be formed through the DNA hybridization. With this technique, it would be possible to create three-dimensional hybrid structures of pancreatic islets coated with various accessory cells, such as patients’ own cells, mesenchymal and adipose-derived stem cells, endothelial progenitor cells, neural crest stem cells or regulatory T cells, which might significantly improve the outcome of islet transplantation in diabetic patients.