کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2022339 1542365 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Safety assessment of bone marrow derived MSC grown in platelet-rich plasma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Safety assessment of bone marrow derived MSC grown in platelet-rich plasma
چکیده انگلیسی


• Human bone marrow-derived mesenchymal stem cells cultured with own platelet rich plasma.
• The angiogenetic effect of cultured human BM-MSC.
• Safety evaluation of cultured human BM-MSC.

The injection of endothelial progenitor cells and mononuclear cells derived from bone marrow at the ischemic region of peripheral artery disease patients is reported to be effective for therapeutic angiogenesis; however, these cell therapies require large amounts of bone marrow to obtain sufficient numbers of cells. To solve this problem, we attempted to culture bone-marrow-derived mesenchymal stem cells (BM-MSC), which are supposed to secrete several cytokines that promote angiogenesis. We also focused on using platelet-rich plasma (PRP) as a supplement for cell culture instead of fetal bovine serum. Human BM-MSC obtained from healthy volunteers expanded rapidly when cultured with 10% PRP prepared from their own blood. FACS analysis revealed that these cultured human MSC were homogeneous populations, and chromosomal analysis showed a normal karyotype. Moreover, the angiogenetic effect was apparent two weeks after human BM-MSC were injected into the ischemic muscle in SCID mice. Tumor formation was not detected three months after injection into SCID mice either subcutaneously or intramuscularly. To simulate clinical settings, canine BM-MSC were grown with canine PRP and injected into their ischemic muscles. We confirmed that donor cells existed in situ two and six weeks after operation without any side effects. These results suggest that cultured human BM-MSC can be a promising cell source for therapeutic angiogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regenerative Therapy - Volume 1, June 2015, Pages 72–79
نویسندگان
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