کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2023006 | 1542431 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interleukin-1Ã induces the novel adipokine chemerin in adipocytes in vitro
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Chemerin has recently been characterized as a novel adipokine playing a crucial role in adipocyte differentiation and insulin signalling. In the current study, the impact of insulin resistance-inducing and proinflammatory interleukin (IL)-1à on chemerin protein secretion and mRNA expression was determined in 3T3-L1 adipocytes. Interestingly, IL-1à significantly induced chemerin protein secretion almost 1.3-fold from 5.89 ng/ml (basal) to 7.52 ng/ml. Furthermore, chemerin mRNA synthesis was significantly stimulated by IL-1à in a dose-dependent fashion with 1.5-fold induction seen at IL-1à concentrations as low as 0.07 ng/ml and maximal 2.6-fold upregulation found at 2 ng/ml effector. Induction of chemerin mRNA by IL-1à was time-dependent in both 3T3-L1 adipocytes and brown fat cells. Signalling studies suggested that Janus kinase 2, nuclear factor κ B, p44/42 mitogen-activated protein kinase, and phosphatidylinositol 3-kinase are involved in IL-1Ã-induced chemerin mRNA expression. Furthermore, recombinant chemerin downregulated insulin-stimulated glucose uptake. Taken together, we show that chemerin is upregulated in fat cells by IL-1à and might modulate the effects of IL-1à on adipocyte metabolism and insulin sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 154, Issues 1â3, 10 April 2009, Pages 102-106
Journal: Regulatory Peptides - Volume 154, Issues 1â3, 10 April 2009, Pages 102-106
نویسندگان
Susan Kralisch, Sebastian Weise, Grit Sommer, Jana Lipfert, Ulrike Lossner, Matthias Bluher, Michael Stumvoll, Mathias Fasshauer,