کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2023449 | 1542447 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Melanocortin-3 receptor activates MAP kinase via PI3 kinase Melanocortin-3 receptor activates MAP kinase via PI3 kinase](/preview/png/2023449.png)
HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10− 10–10− 6 M). ERK1/2 was phosphorylated in a dose-dependent manner with an EC50 of 3.3 ± 1.5 × 10− 9 M, similar to the IC50 of NDP-MSH binding to the MC3R. ERK1/2 phosphorylation was blocked by the melanocortin receptor antagonists SHU9119. NDP-MSH-induced ERK1/2 phosphorylation was sensitive to pertussis toxin and the PI3K inhibitor, wortmannin. Rp-cAMPS, BAPTA-AM and Myr-PKC did not inhibit the NDP-MSH-induced ERK1/2 phosphorylation. NDP-MSH stimulated cellular proliferation in a dose-dependent manner with a similar EC50 to ERK1/2 phosphorylation, 2.1 ± 0.6 × 10− 9 M. Cellular proliferation was blocked by AGRP (86–132) and by the MEK inhibitor, PD98059. The NDP-MSH did not inhibit serum deprivation-induced apoptosis. MC3R activation induces ERK1/2 phosphorylation via PI3K and this pathway is involved in cellular proliferation in HEK cells expressing MC3R.
Journal: Regulatory Peptides - Volume 139, Issues 1–3, 1 March 2007, Pages 115–121