کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2025741 | 1070008 | 2007 | 4 صفحه PDF | دانلود رایگان |

This study used inoculated, artificial soil microcosms containing sand, clay, cellulose, and localized “hotspots” of highly labile, organic N-containing dead bacteria to study N mineralization and assimilation at millimeter scales. Labeling with 15NH4+ along with measurement of its assimilation into microbial biomass at the bulk scale allowed estimation of gross rates of ammonification and N assimilation using isotope dilution. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) analyses of transects of organic-15N across Si wafers in contact with the microcosms indicated strong gradients of 15NH4+ assimilation as a function of proximity to the hotspots that were not apparent using bulk analyses. This combination of bulk and ToF-SIMS analyses represents a powerful approach to explore the physical and biochemical factors that affect N process heterogeneities in soils.
Journal: Soil Biology and Biochemistry - Volume 39, Issue 3, March 2007, Pages 823–826