کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2029891 1070989 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Structure of PknB Extracellular PASTA Domain from Mycobacterium tuberculosis Suggests a Ligand-Dependent Kinase Activation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The Structure of PknB Extracellular PASTA Domain from Mycobacterium tuberculosis Suggests a Ligand-Dependent Kinase Activation
چکیده انگلیسی

SummaryPknB is a transmembrane Ser/Thr protein kinase that defines and belongs to an ultraconserved kinase subfamily found in Gram-positive bacteria. Essential for Mycobacterium tuberculosis growth, its close homolog in Bacillus subtilis has been linked to exit from dormancy. The kinase possesses an extracellular region composed of a repetition of PASTA domains, believed to bind peptidoglycan fragments that might act as a signaling molecule. We report here the first solution structure of this extracellular region. Small-angle X-ray scattering and nuclear magnetic resonance studies show that the four PASTA domains display an unexpected linear organization, contrary to what is observed in the distant protein PBP2x from Streptococccus pneumoniae where two PASTA domains fold over in a compact structure. We propose a model for PknB activation based on a ligand-dependent dimerization of the extracellular PASTA domains that initiates multiple signaling pathways.


► The Ser/Thr protein kinase PknB posses an external domain composed of four PASTA modules
► PknB Bacillus subtilis homolog is a receptor that promotes the exit of dormancy
► First structure of the kinase external PASTA domain
► Unexpected linear organization for the modules suggest ligand-dependent dimerization

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 18, Issue 5, 12 May 2010, Pages 606–615
نویسندگان
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