کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2029994 1071009 2010 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural and Dynamical Insights into the Opening Mechanism of P. aeruginosa OprM Channel
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structural and Dynamical Insights into the Opening Mechanism of P. aeruginosa OprM Channel
چکیده انگلیسی

SummaryOriginally described in bacteria, drug transporters are now recognized as major determinants in antibiotics resistance. For Gram-negative bacteria, the reversible assembly consisting of an inner membrane protein responsible for the active transport, a periplasmic protein, and an exit outer membrane channel achieves transport. The opening of the outer membrane protein OprM from Pseudomonas aeruginosa was modeled through normal mode analysis starting from a new X-ray structure solved at 2.4 Å resolution in P212121 space group. The three monomers are not linked by internal crystallographic symmetries highlighting the possible functional differences. This structure is closed at both ends, but modeling allowed for an opening that is not reduced to the classically proposed “iris-like mechanism.”

Graphical AbstractFigure optionsDownload high-quality image (240 K)Download as PowerPoint slideHighlights
► A trimer OprM protein structure without three-fold crystallographic axis
► Model of OprM opening by normal mode analysis

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 18, Issue 4, 14 March 2010, Pages 507–517
نویسندگان
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