Structural and Dynamical Insights into the Opening Mechanism of P. aeruginosa OprM Channel

SummaryOriginally described in bacteria, drug transporters are now recognized as major determinants in antibiotics resistance. For Gram-negative bacteria, the reversible assembly consisting of an inner membrane protein responsible for the active transport, a periplasmic protein, and an exit outer membrane channel achieves transport. The opening of the outer membrane protein OprM from Pseudomonas aeruginosa was modeled through normal mode analysis starting from a new X-ray structure solved at 2.4 Å resolution in P212121 space group. The three monomers are not linked by internal crystallographic symmetries highlighting the possible functional differences. This structure is closed at both ends, but modeling allowed for an opening that is not reduced to the classically proposed “iris-like mechanism.”

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► A trimer OprM protein structure without three-fold crystallographic axis
► Model of OprM opening by normal mode analysis