کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2030147 1071042 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal Structure of the Cytoplasmic Domain of the Chloride Channel ClC-0
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Crystal Structure of the Cytoplasmic Domain of the Chloride Channel ClC-0
چکیده انگلیسی

SummaryIon channels are frequently organized in a modular fashion and consist of a membrane-embedded pore domain and a soluble regulatory domain. A similar organization is found for the ClC family of Cl− channels and transporters. Here, we describe the crystal structure of the cytoplasmic domain of ClC-0, the voltage-dependent Cl− channel from T. marmorata. The structure contains a folded core of two tightly interacting cystathionine β-synthetase (CBS) subdomains. The two subdomains are connected by a 96 residue mobile linker that is disordered in the crystals. As revealed by analytical ultracentrifugation, the domains form dimers, thereby most likely extending the 2-fold symmetry of the transmembrane pore. The structure provides insight into the organization of the cytoplasmic domains within the ClC family and establishes a framework for guiding future investigations on regulatory mechanisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 14, Issue 2, 2 February 2006, Pages 299–307
نویسندگان
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