کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2030366 1071090 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rounding up: Engineering 12-Membered Rings from the Cyclic 11-Mer TRAP
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Rounding up: Engineering 12-Membered Rings from the Cyclic 11-Mer TRAP
چکیده انگلیسی

SummaryThe protein TRAP (trp RNA binding attenuation protein) forms a highly thermostable ring-shaped 11-mer. By linking in tandem two, three, or four DNA sequences encoding TRAP monomers, we have engineered new rings that consist of 12 TRAP subunits and bind 12 ligand molecules. The hydrogen bonding pattern and buried surface area within and between subunits are essentially identical between the 11-mer and 12-mer crystal structures. Why do the artificial proteins choose to make single 12-mer rings? The 12-mer rings are highly sterically strained by their peptide linkers and far from thermostable. That proteins choose to adopt a strained conformation of few subunits rather than an unstrained one with 11 subunits demonstrates the importance of entropic factors in controlling protein-protein interactions in general.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 14, Issue 5, May 2006, Pages 925–933
نویسندگان
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