کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2030643 | 1071226 | 2015 | 9 صفحه PDF | دانلود رایگان |
• We define the key structural features shared by CRISPR-associated endoRNases.
• We explain how Cas6 enzymes recognize RNA with high affinity and specificity.
• We review the cleavage mechanisms of different CRISPR-associated endoribonucleases.
• We describe the roles of Cas5 and Cas6 proteins in CRISPR interference complexes.
• We discuss future work and development of Cas6-based biotechnological applications.
Many bacteria and archaea possess an adaptive immune system consisting of repetitive genetic elements known as clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins. Similar to RNAi pathways in eukaryotes, CRISPR–Cas systems require small RNAs for sequence-specific detection and degradation of complementary nucleic acids. Cas5 and Cas6 enzymes have evolved to specifically recognize and process CRISPR-derived transcripts into functional small RNAs used as guides by interference complexes. Our detailed understanding of these proteins has led to the development of several useful Cas6-based biotechnological methods. Here, we review the structures, functions, mechanisms, and applications of the enzymes responsible for CRISPR RNA (crRNA) processing, highlighting a fascinating family of endonucleases with exquisite RNA recognition and cleavage activities.
Journal: - Volume 40, Issue 1, January 2015, Pages 58–66