کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2037886 1072341 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells
چکیده انگلیسی

SummaryWe describe the results of a genome-wide analysis of human cells that suggests that most protein-coding genes, including most genes thought to be transcriptionally inactive, experience transcription initiation. We found that nucleosomes with H3K4me3 and H3K9,14Ac modifications, together with RNA polymerase II, occupy the promoters of most protein-coding genes in human embryonic stem cells. Only a subset of these genes produce detectable full-length transcripts and are occupied by nucleosomes with H3K36me3 modifications, a hallmark of elongation. The other genes experience transcription initiation but show no evidence of elongation, suggesting that they are predominantly regulated at postinitiation steps. Genes encoding most developmental regulators fall into this group. Our results also identify a class of genes that are excluded from experiencing transcription initiation, at which mechanisms that prevent initiation must predominate. These observations extend to differentiated cells, suggesting that transcription initiation at most genes is a general phenomenon in human cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 130, Issue 1, 13 July 2007, Pages 77–88
نویسندگان
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