کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2043922 1073382 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MicroRNA Function Is Globally Suppressed in Mouse Oocytes and Early Embryos
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
MicroRNA Function Is Globally Suppressed in Mouse Oocytes and Early Embryos
چکیده انگلیسی

SummaryDicer, which is required for the processing of both microRNAs (miRNAs) and small interfering RNAs (siRNAs), is essential for oocyte maturation 1 and 2. Oocytes express both miRNAs and endogenous siRNAs (endo-siRNAs) 3 and 4. To determine whether the abnormalities in Dicer knockout oocytes during meiotic maturation are secondary to the loss of endo-siRNAs and/or miRNAs, we deleted Dgcr8, which encodes an RNA-binding protein specifically required for miRNA processing. In striking contrast to Dicer, Dgcr8-deficient oocytes matured normally and, when fertilized with wild-type sperm, produced healthy-appearing offspring, even though miRNA levels were reduced to similar levels as Dicer-deficient oocytes. Furthermore, the deletion of both maternal and zygotic Dgcr8 alleles did not impair preimplantation development, including the determination of the inner cell mass and trophectoderm. Most surprisingly, the mRNA profiles of wild-type and Dgcr8 null oocytes were essentially identical, whereas Dicer null oocytes showed hundreds of misregulated transcripts. These findings show that miRNA function is globally suppressed during oocyte maturation and preimplantation development and that endo-siRNAs, rather than miRNAs, underlie the Dicer knockout phenotype in oocytes.

Graphical AbstractFigure optionsDownload high-quality image (230 K)Download as PowerPoint slideHighlights
► MicroRNA function is globally suppressed in mature oocytes and early embryos
► Endo-siRNAs, not miRNAs, are essential for oocyte maturation
► Endo-siRNA and microRNA function are separable downstream of Dicer maturation

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 20, Issue 3, 9 February 2010, Pages 271–277
نویسندگان
, , , , , , ,