کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048326 | 1074076 | 2012 | 5 صفحه PDF | دانلود رایگان |

MIZ1 is encoded by a gene essential for root hydrotropism in Arabidopsis. To characterize the property of MIZ1, we used transgenic plants expressing GFP-tagged MIZ1 (MIZ1-GFP) and mutant MIZ1 (MIZ1G235E-GFP) in a miz1-1 mutant. Although both chimeric genes were transcribed, the translational products of MIZ1G235E-GFP did not accumulate in roots. Moreover, MIZ1-GFP complemented the mutant phenotype but not MIZ1G235E-GFP. The signal corresponding to MIZ1-GFP was detected at high levels in cortical cells and lateral root cap cells and accumulated in compartments in cortical cells. MIZ1-GFP was fractionated into a soluble protein fraction and an endoplasmic reticulum (ER) membrane fraction, where it was bound to the surface of the ER membrane at the cytosolic side.
► MIZ1 is a previously uncharacterized protein encoded by a gene essential for root hydrotropism.
► GFP-tagged MIZ1 is associated with the endoplasmic reticulum membrane (ER) at the cytosolic side.
Journal: FEBS Letters - Volume 586, Issue 4, 17 February 2012, Pages 398–402