کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048326 1074076 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis root cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis root cells
چکیده انگلیسی

MIZ1 is encoded by a gene essential for root hydrotropism in Arabidopsis. To characterize the property of MIZ1, we used transgenic plants expressing GFP-tagged MIZ1 (MIZ1-GFP) and mutant MIZ1 (MIZ1G235E-GFP) in a miz1-1 mutant. Although both chimeric genes were transcribed, the translational products of MIZ1G235E-GFP did not accumulate in roots. Moreover, MIZ1-GFP complemented the mutant phenotype but not MIZ1G235E-GFP. The signal corresponding to MIZ1-GFP was detected at high levels in cortical cells and lateral root cap cells and accumulated in compartments in cortical cells. MIZ1-GFP was fractionated into a soluble protein fraction and an endoplasmic reticulum (ER) membrane fraction, where it was bound to the surface of the ER membrane at the cytosolic side.


► MIZ1 is a previously uncharacterized protein encoded by a gene essential for root hydrotropism.
► GFP-tagged MIZ1 is associated with the endoplasmic reticulum membrane (ER) at the cytosolic side.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 586, Issue 4, 17 February 2012, Pages 398–402
نویسندگان
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