کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048805 1074101 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel secreted metzincin metalloproteinase from Bacillus intermedius
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A novel secreted metzincin metalloproteinase from Bacillus intermedius
چکیده انگلیسی

The mprBi gene from Bacillus intermedius 3–19 encoding a novel secreted metalloproteinase was identified. The mpriBi gene was expressed in an extracellular proteinase-deficient Bacillus subtilis BG 2036 strain and the corresponding protein was characterized biochemically. The 19 kDa MprBi protein was purified to homogeneity and sequenced by mass spectroscopy and Edman degradation methods. Amino acid sequence analysis of MprBi identified an active site motif HEYGHNFGLPHD and a conserved structural component Met-turn, both of which are unique features of the metzincin clan. Furthermore, MprBi harbors a number of distinct sequence elements characteristic of proteinase domains in eukaryotic adamalysins. We conclude that MprBi and similar proteins from other Bacillus species form a novel group of metzincin metalloproteinases in prokaryotes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 21, 5 November 2010, Pages 4419–4425
نویسندگان
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