کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048972 | 1074108 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Engineered epidermal growth factor mutants with faster binding on-rates correlate with enhanced receptor activation Engineered epidermal growth factor mutants with faster binding on-rates correlate with enhanced receptor activation](/preview/png/2048972.png)
Receptor tyrosine kinases (RTKs) regulate critical cell signaling pathways, yet the properties of their cognate ligands that influence receptor activation are not fully understood. There is great interest in parsing these complex ligand–receptor relationships using engineered proteins with altered binding properties. Here we focus on the interaction between two engineered epidermal growth factor (EGF) mutants and the EGF receptor (EGFR), a model member of the RTK superfamily. We found that EGF mutants with faster kinetic on-rates stimulate increased EGFR activation compared to wild-type EGF. These findings support previous predictions that faster association rates correlate with enhanced receptor activity.Structured summary of protein interactionshumanEGFRbinds to humanEGF by surface plasmon resonance (View Interaction 1, 2, 3)mouseEGFRbinds to humanEGF by surface plasmon resonance (View Interaction 1, 2, 3)humanEGFRphysically interacts with humanEGF by fluorescence-activated cell sorting (View Interaction 1, 2, 3, 4, 5, 6, 7)
Journal: FEBS Letters - Volume 585, Issue 8, 20 April 2011, Pages 1135–1139