کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2049162 1074118 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mutant p62/SQSTM1 UBA domains linked to Paget’s disease of bone differ in their abilities to function as stabilization signals
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Mutant p62/SQSTM1 UBA domains linked to Paget’s disease of bone differ in their abilities to function as stabilization signals
چکیده انگلیسی

We show that the ubiquitin-associated domain (UBA) of human p62/sequestosome-1 (SQSTM1) can delay degradation of proteasome substrates in yeast. Taking advantage of naturally occurring mutant UBA domains that are linked to Paget’s disease of bone (PDB), we found that three of the four mutant UBA domains tested in this study were able to inhibit proteasomal degradation, albeit not to the same extent as the wild-type domain. Interestingly, the stability measured as the fraction of folded protein, and not the ubiquitin binding properties, of the PDB-associated UBA domains correlated with their protective effects. These data suggest that the protective effect of UBA domains depends on their structural integrity rather than ubiquitin binding capabilities.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 8, 16 April 2010, Pages 1585–1590
نویسندگان
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