کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2049657 1074137 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The cytosolic subunit p67phox of the NADPH-oxidase complex does not bind NADPH
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The cytosolic subunit p67phox of the NADPH-oxidase complex does not bind NADPH
چکیده انگلیسی

The NADPH-oxidase of phagocytic cells is a multicomponent enzyme that generates superoxide. It comprises a membrane flavocytochrome b558 and four cytosolic proteins; p67phox, p47phox, p40phox and Rac. The NADPH-binding site of this complex was shown to be located on the flavocytochrome b558. However, a number of studies have suggested the presence of another site on the p67phox subunit which is the key activating component. Using several approaches like tryptophan quenching fluorescence measurement, inhibition by 2′,3′-dialdehyde NADPH, and free/bound NADPH concentration measurements, we demonstrate that no NADPH binds on p67phox, thus definitively solving the controversy on the number and location of the NADPH-binding sites on this complex.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 19, 6 October 2009, Pages 3225–3229
نویسندگان
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