SHIP2 associates with intersectin and recruits it to the plasma membrane in response to EGF

We identified intersectin1 (ITSN1) as a new binding partner of the SH2 domain containing inositol 5-phosphatase 2 (SHIP2). The interaction between SHIP2 and ITSN1 was confirmed in vivo. Src homology 3D, A, C, and E domains of ITSN1 were shown to be implicated in the interaction. In response to epidermal growth factor, SHIP2 expression could recruit the ITSN1 short form (ITSN1-S) to the cell membrane, while SHIP2 overexpression did not modulate the ITSN-mediated extracellular signal-regulated kinase1/2 and c-Jun NH2-terminal kinase activation. Our data provide a molecular link between SHIP2 and ITSN1 which are involved in receptor endocytosis regulation.Structured summaryMINT-6673604:ITSN-1S (uniprotkb:Q15811-2) and SHIP2 (uniprotkb:O15357) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6673646, MINT-6694843:SHIP2 (uniprotkb:Q6P549) physically interacts (MI:0218) with ITSN-1S (uniprotkb:Q9Z0R4-2) by anti bait coimmunoprecipitation (MI:0006)MINT-6673386:SHIP2 (uniprotkb:O15357) physically interacts (MI:0218) with ITSN-1S (uniprotkb:Q15811-2) by two hybrid (MI:0018)MINT-6673429, MINT-6673447, MINT-6673475:ITSN-1S (uniprotkb:Q15811-2) physically interacts (MI:0218) with SHIP2 (uniprotkb:O15357) by anti tag coimmunoprecipitation (MI:0007)MINT-6673513, MINT-6673542, MINT-6673557, MINT-6673572:ITSN-1S (uniprotkb:Q15811-2) physically interacts (MI:0218) with SHIP2 (uniprotkb:O15357) by pull down (MI:0096)MINT-6673462:ITSN-1L (uniprotkb:Q15811-1) physically interacts (MI:0218) with SHIP2 (uniprotkb:O15357) by anti tag coimmunoprecipitation (MI:0007)