کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050068 | 1074152 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: New tensio-active molecules stabilize a human G protein-coupled receptor in solution New tensio-active molecules stabilize a human G protein-coupled receptor in solution](/preview/png/2050068.png)
Structural characterization of membrane proteins is hampered by the instability of the isolated proteins in detergent solutions. Here, we describe a new class of phospholipid-like surfactants that stabilize the G protein-coupled receptor, BLT1. These compounds, called C13U9, C13U19, C15U25 and C17U16, were synthesized by radical polymerization of Tris(hydroxymethyl) acrylamidomethane in the presence of thioglycerol, first endowed with two hydrocarbon chains with variable lengths (13–17 carbon atoms), as transfer reagent. C13U19, C17U16 or C15U25 significantly enhanced the stability of BLT1 in solution compared to what was obtained with common detergents. These molecules therefore represent a promising step towards the structural characterization of BLT1 and possibly other membrane proteins.
Journal: FEBS Letters - Volume 581, Issue 10, 15 May 2007, Pages 1944–1950