کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050080 1074152 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi
چکیده انگلیسی

The Trypanosoma cruzi karyotype shows an extensive chromosomal size polymorphism. Absence of condensed mitotic chromosomes and chromatin fragility are characteristic features of T. cruzi which would allow DNA breaks and chromosomal rearrangements during cell proliferation. We have investigated by pulsed field gel electrophoresis (PFGE) eventual changes in chromosomal size during exponential and stationary phases of T. cruzi epimastigotes in culture, in G0 trypomastigotes and throughout the cell cycle in synchronized epimastigotes. T. cruzi molecular karyotype was stable throughout the cell cycle and during differentiation. Thus, the chromosomal size polymorphism previously reported in T. cruzi contrasts with the stability of the molecular karyotype observed here and suggests that chromosomal rearrangements leading to changes in chromosomal size are scarce events during the clonal propagation of this parasite.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 10, 15 May 2007, Pages 2022–2026
نویسندگان
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