کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050685 | 1074177 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A HIF-1alpha-related gene involved in cell protection from hypoxia by suppression of mitochondrial function A HIF-1alpha-related gene involved in cell protection from hypoxia by suppression of mitochondrial function](/preview/png/2050685.png)
Recently, we reported that acetylcholine-induced hypoxia-inducible factor-1α protects cardiomyocytes from hypoxia; however, the downstream factors reducing hypoxic stress are unknown. We identified apoptosis inhibitor (AI) gene as being differentially expressed between von Hippel Lindau (VHL) protein-positive cells with high levels of GRP78 expression and VHL-negative cells with lower GRP levels, using cDNA subtraction. AI decreased GRP78 level, suppressed mitochondrial function, reduced oxygen consumption and, ultimately, suppressed hypoxia-induced apoptosis. By contrast, knockdown of the AI gene increased mitochondrial function. Hypoxic cardiomyocytes and ischemic myocardium showed increased AI mRNA expression. These findings suggest that AI is involved in suppressing mitochondrial function, thereby leading to cellular stress eradication and consequently to protection during hypoxia.
Journal: FEBS Letters - Volume 582, Issue 2, 23 January 2008, Pages 332–340