کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050974 1074187 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases
چکیده انگلیسی

Plant legumains are cysteine proteinases putatively involved in processing endogenous proteins. Phytocystatins (PhyCys) have been described as plant inhibitors of papain-like cysteine proteinases. Some PhyCys contain a carboxy terminal extension with an amino acid motif (SNSL) similar to that involved in the inhibition of legumain-like proteins by human cystatins. The role of these carboxy terminal extended PhyCys as inhibitors of legumain-like cysteine proteinases is here shown by in vitro inhibition of human legumain and legumain-like activities from barley extracts. Moreover, site-directed mutagenesis has demonstrated that the asparagine of the SNSL motif is essential in this inhibition. We prove for first time the existence of legumain inhibitors in plants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 16, 26 June 2007, Pages 2914–2918
نویسندگان
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