کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050991 1074187 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Contributions of Zn(II)-binding to the structural stability of endostatin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Contributions of Zn(II)-binding to the structural stability of endostatin
چکیده انگلیسی

Endostatin has a compact structure with a Zn(II)-binding site (His1, His3, His11, and Asp76) at the N-terminus. In this study, the effects of Zn(II)-binding on the folding and stability of recombinant human endostatin were studied. The results show that Zn(II)-binding largely stabilizes the structure of endostatin at physiological pH. Under some proteolytic conditions, Zn(II)-binding also contributes to the integrity of the N-terminus of endostatin, which is critical for endostatin to maintain a stable structure. Moreover, engineering an extra Zn(II)-binding peptide to the N-terminus of human endostatin makes this molecule more stable and cooperative in the presence of Zn(II).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 16, 26 June 2007, Pages 3027–3032
نویسندگان
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