کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051345 1074197 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The parkinsonism producing neurotoxin MPP+ affects microtubule dynamics by acting as a destabilising factor
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The parkinsonism producing neurotoxin MPP+ affects microtubule dynamics by acting as a destabilising factor
چکیده انگلیسی

Dysfunction of the microtubule system is emerging as a contributing factor in a number of neurodegenerative diseases. Looking for the potential role played by the microtubule cytoskeleton in neuron degeneration underlying Parkinson’s disease (PD), we investigate the influence of the parkinsonism producing neurotoxin 1-methyl-4-phenylpyridinium (MPP+) on microtubule dynamics. We find that it acts as a strong catastrophe promoter causing a decrease of the average length of microtubules assembled from purified tubulin. We also find that it reduces the number of microtubules nucleated from purified centrosomes. Finally, binding assays demonstrate that the neurotoxin binds specifically to tubulin in the microtubule lattice in a close to stoichiometric manner. This paper provides the first evidence that dynamic instability of microtubules is specifically affected by MPP+ and suggests that it could play a role in neuronal cell death underlying PD.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 21, 29 August 2005, Pages 4781–4786
نویسندگان
, , ,