کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051948 1074217 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Compact oleic acid in HAMLET
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Compact oleic acid in HAMLET
چکیده انگلیسی

HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 27, 7 November 2005, Pages 6095–6100
نویسندگان
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