کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2053127 1074260 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of the osteoclast V-ATPase by small interfering RNAs
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Inhibition of the osteoclast V-ATPase by small interfering RNAs
چکیده انگلیسی

The multisubunit enzyme V-ATPase harbours isoforms of individual subunits. a3 is one of four 116 kDa subunit a isoforms, and it is crucial for bone resorption. We used small interfering RNA (siRNA) molecules to knock down a3 in rat osteoclast cultures. Labeled siRNA-molecules entered osteoclasts via endocytosis and knocked down the a3 mRNA. Bone resorption was decreased in siRNA-treated samples due to decreased acidification and osteoclast inactivation. Expression of a1 did not respond to decreased a3 levels, suggesting that a1 does not compensate for a3 in osteoclast cultures. Subunit a3 is thus an interesting target for novel nucleic acid therapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 22, 12 September 2005, Pages 4937–4942
نویسندگان
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