کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2053324 | 1074270 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene expression and characterization of isoprene synthase from Populus alba
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کلمات کلیدی
GFP, green fluorescent protein - GFP، پروتئین فلورسنت سبزGPP, geranyl diphosphate - GPP، ژانایل دی فسفاتIPP, Isopentenyl diphosphate - IPP، Isopentenyl DiphosphateIsoprene synthase - ایزوپرن سیتستازHeterologous expression - بیان هترولوژیکGene expression - بیان ژنSubstrate specificity - خصوصیات زیربناییSubcellular localization - محلی سازی سلولMEP pathway - مسیر MEP
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Gene expression and characterization of isoprene synthase from Populus alba Gene expression and characterization of isoprene synthase from Populus alba](/preview/png/2053324.png)
چکیده انگلیسی
Isoprene synthase cDNA from Populus alba (PaIspS) was isolated by RT-PCR. This PaIspS mRNA, which was predominantly observed in the leaves, was strongly induced by heat stress and continuous light irradiation, and was substantially decreased in the dark, suggesting that isoprene emission was regulated at the transcriptional level. The subcellular localization of PaIspS protein with green fluorescent protein fusion was shown to be in plastids. PaIspS expressed in Escherichia coli was characterized enzymatically: it had an optimum pH of approximately 8.0, and an optimum temperature 40 °C. Its preference for divalent cations for its activity was also studied.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 11, 25 April 2005, Pages 2514–2518
Journal: FEBS Letters - Volume 579, Issue 11, 25 April 2005, Pages 2514–2518
نویسندگان
Kanako Sasaki, Kazuaki Ohara, Kazufumi Yazaki,