کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2055813 1075777 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of candidate reference genes for the expression studies with brassinosteroids in Lolium perenne and Triticum aestivum
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Assessment of candidate reference genes for the expression studies with brassinosteroids in Lolium perenne and Triticum aestivum
چکیده انگلیسی

Quantitative PCR studies need proper reference genes with expression stability exclusively validated under certain experimental conditions. The expression stability of several genes commonly used as references was tested under 24-epibrassinolide (EBR) and temperature treatment. Different statistical approaches (qBasePLUS, BestKeeper, NormFinder) were used to prepare rankings of expression stability in two species of an economic importance: common wheat (Triticum aestivum) and perennial ryegrass (Lolium perenne). Candidate reference genes were shown to be regulated differentially in these two plant species. The maximum stability values indicated that the expression stability was higher in T. aestivum. Taking into account of all ranks it seems that TBP-1 and UBI in ryegrass and ACT, ADP and EF1A in wheat should be used as reference genes in the brassinosteroids and temperature involving studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 171, Issue 16, 15 October 2014, Pages 1541–1544
نویسندگان
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