کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2056815 | 1075843 | 2011 | 5 صفحه PDF | دانلود رایگان |
This study investigated the role of the protein kinase MEK1/2 in the regulation of ascorbate and glutathione content by jasmonic acid in Agropyron cristatum leaves. The results showed that JA induced increases in the transcript levels and activities of APX, GR, MDHAR, DHAR, GalLDH and γ-ECS, the contents of AsA, GSH, total ascorbate and total glutathione, and the ratios of AsA/DHA and GSH/GSSG, and reduced the EGSSG/2GSH. The increases, except for the transcript level and activity of γ-ECS and the reduction in EGSSG/2GSH, were all suppressed by pre-treatment with the MEK1/2 inhibitors PD98059 and U0126. The results of western blot analyses showed that JA induced increases in the phosphorylation level of MEK1/2. Our results suggest that JA could induce the activation of MEK1/2 by increasing the phosphorylation level, which, in turn, resulted in the up-regulation of ascorbate and glutathione content in A. cristatum leaves.
Journal: Journal of Plant Physiology - Volume 168, Issue 5, 15 March 2011, Pages 514–518