کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2057278 1075873 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning of Phaseolus vulgaris cDNA encoding proliferating cell nuclear antigen
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Molecular cloning of Phaseolus vulgaris cDNA encoding proliferating cell nuclear antigen
چکیده انگلیسی

SummaryA cDNA fragment encoding a common bean (Phaseolus vulgaris) proliferating cell nuclear antigen (PCNA) was isolated using rapid amplification of cDNA 3′ end (3′ RACE) method, cloned and sequenced. The nucleotide sequence of this clone contains an open reading frame of 798 nucleotides encoding a protein of 265 amino acids. Alignment of the common bean PCNA predicted sequence shows its high degree of identity with PCNA from other plant species. Analysis of PCNA content in the germinating embryos of common bean showed a decrease in the protein level after 60 h of germination. Moreover, PCNA was not detected in the tested plant organs (root, stem, leaf and flower). The presence of PCNA in the germinating seeds and its absence from mature plants suggests that this protein plays a crucial role during early stages of plant development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 164, Issue 2, 23 February 2007, Pages 209–213
نویسندگان
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