کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2057964 | 1076139 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds](/preview/png/2057964.png)
SummaryTwo abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when seeds were artificially dried. FsClo1 codes for a caleosin, a calcium-binding protein. By expressing FsCLO1 as a fusion protein in Escherichia coli, we obtained biochemical evidence of its ability to bind calcium, a second messenger involved in ABA signaling. This cDNA clone is seed specific, shows a LEA expression pattern and is also induced by ABA and dehydration treatments. The relationship of both genes with seed responses to ABA is discussed.
Journal: Journal of Plant Physiology - Volume 165, Issue 17, 28 November 2008, Pages 1798–1807