Examining genetic relationships of Chinese Pleurotus ostreatus cultivars by combined RAPD and SRAP markers

Combined randomly amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) were used to assess the genetic diversity of Pleurotus ostreatus strains cultivated in China. For the RAPD and SRAP analyses, 479 and 282 polymorphic bands were obtained from 20 P. ostreatus strains using 20 and 13 selected primers or primer pairs, respectively. A combined RAPD/SRAP dendrogram grouped the 20 strains into five clades with a coefficient of 0.690. The comparison of RAPD and SRAP was evaluated in the present study. The combined RAPD/SRAP markers provided reliable information regarding the relationships among the P. ostreatus strains.

► An average of 94.85% of polymorphism was obtained using 20 selected RAPD primers.
► An average of 94% of polymorphism was obtained using 13 selected SRAP primer pairs.
► A combined RAPD/SRAP dendrogram grouped the 20 strains into five clades with coefficient of 0.690.
► These results revealed the significant genetic diversity among Chinese Pleurotus ostreatus cultivars.