کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2062145 1076561 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Timing of Perialgal Vacuole Membrane Differentiation from Digestive Vacuole Membrane in Infection of Symbiotic Algae Chlorella vulgaris of the Ciliate Paramecium bursaria
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Timing of Perialgal Vacuole Membrane Differentiation from Digestive Vacuole Membrane in Infection of Symbiotic Algae Chlorella vulgaris of the Ciliate Paramecium bursaria
چکیده انگلیسی

Each symbiotic Chlorella of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole derived from the host digestive vacuole to protect from lysosomal fusion. To understand the timing of differentiation of the perialgal vacuole from the host digestive vacuole, algae-free P. bursaria cells were fed symbiotic C. vulgaris cells for 1.5 min, washed, chased and fixed at various times after mixing. Acid phosphatase activity in the vacuoles enclosing the algae was detected by Gomori's staining. This activity appeared in 3-min-old vacuoles, and all algae-containing vacuoles demonstrated activity at 30 min. Algal escape from these digestive vacuoles began at 30 min by budding of the digestive vacuole membrane into the cytoplasm. In the budded membrane, each alga was surrounded by a Gomori's thin positive staining layer. The vacuoles containing a single algal cell moved quickly to and attached just beneath the host cell surface. Such vacuoles were Gomori's staining negative, indicating that the perialgal vacuole membrane differentiates soon after the algal escape from the host digestive vacuole. This is the first report demonstrating the timing of differentiation of the perialgal vacuole membrane during infection of P. bursaria with symbiotic Chlorella.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protist - Volume 160, Issue 1, February 2009, Pages 65–74
نویسندگان
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