کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2064502 1544139 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Horseradish peroxidase and antibody labeled gold nanoparticle probe for amplified immunoassay of ciguatoxin in fish samples based on capillary electrophoresis with electrochemical detection
ترجمه فارسی عنوان
پراکسیداز هوررودیس و آنتیبادی برچسب پروتئین نانوذره طلای برای آزمایش ایزومتریک سایگواتکسین در نمونه های ماهی مبتنی بر الکتروفورز مویرگی با تشخیص الکتروشیمیایی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی


• Determination of ciguatoxin by simultaneously labeling Ab and HRP on AuNPs.
• High sensitivity obtained by adopting AuNPs as carrier at high HRP/Ab molar ratio.
• The result using CE EC immunoassay for CTX1B were consistent with that of HPLC-MS.

This paper describes a new amplified immunoassay with horseradish peroxidase (HRP) and antibody (Ab) labeled gold nanoparticles (AuNPs) probe hyphenated to capillary electrophoresis (CE) with electrochemical (EC) detection for ultrasensitive determination of ciguatoxin CTX1B. AuNPs were conjugated with HRP and Ab, and then incubated with limited amount of CTX1B to produce immunocomplex. The immunoreactive sample was injected into capillary for CE separation and EC detection. Enhanced sensitivity was obtained by adopting the AuNPs as carriers of HRP and Ab at high HRP/Ab molar ratio. The calibration curve of CTX1B was in the range of 0.06–90 ng/mL. The detection limit was 0.045 ng/mL, which is 38-fold lower than that of HPLC-MS method for CTX1B analysis. The proposed method was successfully applied for the quantification of CTX1B in contamined fish samples by simultaneously labeling Ab and HRP on AuNPs. The amplified IA with HRP and Ab labeled AuNPs probe hyphenated to CE and EC detection provides a sensitive analytical approach for the determination of trace ciguatoxin in complex samples.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 96, 15 March 2015, Pages 89–95
نویسندگان
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