Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH

We report on the technical advance of linearized pDNA (pDNAlinear) above the circular one (pDNAcirc) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNAlinear against poly-l-asparagine indicates that effective ion-pairing occurred between the pDNAlinear and polycations.