کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067329 | 1077893 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Low-dose aspirin promotes endothelial progenitor cell migration and adhesion and prevents senescence
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کلمات کلیدی
COXeNOSEPCsAspirin - آسپرینcyclooxygenase - آنزیم سیکلواکسیژنازProliferation - ترویجEndothelial progenitor cells - سلول های پیش ساز اندوتلیالEndothelial progenitor cell - سلول پیش ساز اندوتلیالendothelial nitric oxide synthase - سنتاز اکسید نیتریک اندوتلیالMigration - مهاجرتPropidium iodide - پروتئین یدیدprostaglandin - پروستاگلاندینهاSenescence - پیریAdhesion - چسبندگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis and endothelial nitric oxide synthase (eNOS) expression. EPC migration was detected by a modified Boyden chamber assay. EPC adhesion assay was performed by counting adherent cells on fibronectin-coated culture dishes. EPC senescence was assessed by both senescence-associated-β-galactosidase staining and DAPI staining. EPC proliferation was analyzed by MTT assay. EPC apoptosis was evaluated by flow cytometric analysis. eNOS protein expression was measured by Western blotting analysis. Aspirin promoted EPC migratory and adhesive capacity at concentrations between 0.1 and 100 μmol/L and prevented senescence at concentrations between 50 and 100 μmol/L. Meanwhile, aspirin in a range of these concentrations did not affect EPC proliferation, apoptosis or eNOS expression. Our findings indicate that low-dose aspirin promotes migration and adhesion and delays the onset of senescence of EPCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 32, Issue 7, July 2008, Pages 761-768
Journal: Cell Biology International - Volume 32, Issue 7, July 2008, Pages 761-768
نویسندگان
Zhengli Hu, Fumin Zhang, Zhijian Yang, Jinying Zhang, Dingguo Zhang, Naiquan Yang, Yuqing Zhang, Kejiang Cao,