کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067538 | 1077898 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Voltage-dependent potassium channels are involved in staurosporine-induced apoptosis of rat mesenchymal stem cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
The strategy of mesenchymal stem cells (MSCs) transplantation is limited by the inability to deliver a large number of grafted cells that resist peri-transplantation apoptosis in ischemic tissues, and this led us to investigate methods of improving the viability of these cells. We demonstrate the presence of voltage-gated potassium channels in rat MSCs that can be activated by staurosporine (ST). MSCs exposed to ST underwent apoptotic cell changes. Tetraethylammonium (TEA), a classic blocker of K+ channels, blocked the ST-induced augmentation of K+ currents, and reduced ST-induced apoptosis. Furthermore, we found that TEA prevented the ST-induced increase in expression of the pro-apoptotic protein Bax and decrease of the anti-apoptotic protein Bcl-2. Taken together, our findings suggest that voltage-gated potassium is involved in ST-induced apoptosis of rat MSCs. TEA blocks the ST-induced augmentation of K+ currents, alters the expression of Bcl-2 family proteins induced by ST, and attenuates the apoptosis of rat MSCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 32, Issue 2, February 2008, Pages 312-319
Journal: Cell Biology International - Volume 32, Issue 2, February 2008, Pages 312-319
نویسندگان
Shaoping Wang, Jian-an Wang, Jun Li, Jinghong Zhou, Hai Wang,