کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067641 | 1077902 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line](/preview/png/2067641.png)
چکیده انگلیسی
We assessed aging in continuous donor skin fibroblast cell line GGM5 up to the 25th passage by in vitro replicative senescence, telomere dynamics and chromosomal abnormalities. Cell proliferation rate increased from 0.84 ± 0.26 (primary cells) to 1.20 ± 0.17 (13-15 passage group) per day and reduced to 0.65 ± 0.14 in 22-25 passages. Cell proliferation rate was reduced by 45.7% after 87.62 CPDs. Cell viability reduced from 100% to 97.4% up to the 25th passages. Frequency of β gal+ cells increased in successive passages and days in culture. The correlation coefficient between frequency of β gal+ cells and growth rate was â0.50 to â0.61. Loss of mean TRF length was 13.8 nucleotides (passage 15) to 95.4 nucleotides per cell division in later passages. All cells showed Robertsonian translocation in 22-25 passaged cells. The SCNT pre-implantation embryos production was highest (22.5%) in donor cells used from 10-15 passages as compared to early (â¤5) and late (22-25 passages). Our findings supports that cell proliferation rates, β gal staining, mean TRF loss and karyological profile are useful marker for evaluation of competent nuclear donor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 10, October 2007, Pages 1257-1264
Journal: Cell Biology International - Volume 31, Issue 10, October 2007, Pages 1257-1264
نویسندگان
Neelam Gupta, Ritu Taneja, Alok Pandey, Manishi Mukesh, Hari Singh, S.C. Gupta,