کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2069563 | 1078399 | 2013 | 6 صفحه PDF | دانلود رایگان |
Anti- and pro-apoptotic Bcl-2 family members regulate the mitochondrial phase of apoptotic cell death. The mitochondrial targeting mechanisms of Bcl-2 family proteins are tightly regulated. Known outer mitochondrial membrane targeting sequences include the C-terminal tail and central helical hairpin. Bcl-xL also localizes to the inner mitochondrial membrane, but these targeting sequences are unknown. Here we investigate the possibility that the N-terminus of Bcl-xL also contains mitochondrial targeting information. Amino acid residues 1–28 of Bcl-xL fused to EGFP are sufficient to target mitochondria. Although positive charges and helical propensity are required for targeting, similar to import sequences the N-terminus is not sufficient for efficient mitochondrial import.
► N-terminal helix of Bcl-xL is sufficient for mitochondrial targeting.
► Residues 1-28 of Bcl-xL fused to EGFP compete with mito-RFP on some mitochondria.
► Helical and basic residues required for targeting are not sufficient for import.
Journal: Mitochondrion - Volume 13, Issue 2, March 2013, Pages 119–124