کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2071382 1078744 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nonviral gene transfer as a tool for studying transcription regulation of xenobiotic metabolizing enzymes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Nonviral gene transfer as a tool for studying transcription regulation of xenobiotic metabolizing enzymes
چکیده انگلیسی

Numerous xenobiotic metabolizing enzymes are regulated by nuclear receptors at transcriptional level. The challenge we currently face is to understand how a given nuclear receptor interacts with its xenobiotics, migrates into nucleus, binds to the xenobiotic response element of a target gene, and regulates transcription. Toward this end, new methods have been developed to introduce the nuclear receptor gene into appropriate cells and study its activity in activating reporter gene expression under the control of a promoter containing xenobiotic response elements. The goal of this review is to critically examine the gene transfer methods currently available. We concentrate on the gene transfer mechanism, advantages and limitations of each method when employed for nuclear receptor-mediated gene regulation studies. It is our hope that the information provided highlights the importance of gene transfer in studying the mechanisms by which our body eliminates the potentially harmful substances and maintains the homeostasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Advanced Drug Delivery Reviews - Volume 62, Issue 13, 30 October 2010, Pages 1250–1256
نویسندگان
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