کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2072472 | 1544707 | 2016 | 6 صفحه PDF | دانلود رایگان |
The present study aimed to determine the effect of different concentrations of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on post-thaw stallion sperm quality. The ejaculates collected from four healthy mature Turkmen stallions were pooled and divided into eight aliquots. The samples were diluted with extenders containing different concentrations (0.5, 1 or 2 mM/mL) of BHA or BHT. The positive control (PC) samples were diluted with extender containing 0.5% ethanol (v/v) whereas; the negative control (NC) samples were diluted with basic extender only. Semen samples were frozen according to a standard protocol. After thawing of samples, sperm motility, viability, membrane integrity, total abnormality and lipid peroxidation were assessed. The greatest (P < 0.05) values for total sperm motility, viability and plasma membrane functionality and least values for malonedialdehyde (MDA) concentration were observed in samples supplemented either with 1 mM BHT or 2 mM BHA. However, the progressive motility was greater (P < 0.05) only in samples treated with 2 mM BHA. In conclusion, the use of 1 mM BHT or 2 mM BHA in extender improves the freezing capacity of stallion sperm by reducing oxidative stress during freeze-thaw process.
Journal: Animal Reproduction Science - Volume 170, July 2016, Pages 108–113