Identification of molecular species of polyol oils produced from soybean oil by Pseudomonas aeruginosa E03-12 NRRL B-59991

The objective of this study is to identify the chemical species of the polyol oil products produced from soybean oil by Pseudomonas aeruginosa E03-12 NRRL B-59991. We reported earlier the polyol products produced from soybean oil by Acinetobacter haemolyticus A01-35 (NRRL B-59985) ( Hou and Lin, 2013). The polyol oil produced by strain A01-35 were a mixture of 57 molecular species of DAG containing tri-, di-, mono-hydroxy FA and normal FA. In this study, tricaprylin was selected as internal standard for HPLC quantitative estimation of products. A CombiFlash chromatographic method was established for separation of the polyol oil products. The molecular species of the polyol oils produced from soybean oil by strain E03-12 were identified with HPLC/MS. We identified 41 derivatives of DAG, among them 32 molecular species containing one hydroxy FA and one normal FA, 8 molecular species containing two hydroxy FA without normal FA, and one molecular specie containing two normal FA without hydroxylated FA. The hydroxy FA included mono-, di- and tri-hydroxy FA. Eight molecular species of DAG containing one trihydroxy FA and 14 molecular species of DAG containing one dihydroxy FA. We have also identified 64 molecular species of TAG, among them 13 molecular species containing two hydroxy FA, 42 molecular species containing one hydroxy FA and 9 molecular species containing no hydroxylated FA. This is different from our previous findings with A. haemolyticus A01-35 which produced only DAG polyol oils. E03-12 is a better strain for developing into an industrial bioprocess.