کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2075523 1079339 2012 7 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Degradation of keratin by keratinase and disulfide reductase from Bacillus sp. MTS of Indonesian origin
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موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Degradation of keratin by keratinase and disulfide reductase from Bacillus sp. MTS of Indonesian origin
چکیده انگلیسی

Bacillus sp. MTS isolated from Tangkuban Perahu crater Indonesia was found capable of degrading whole chicken feather effectively. The bacteria produced extracellular alkaline keratinase and disulfide reductase. When grown in feather media, Bacillus sp. MTS produced multi-fractions of both enzymes. The purified enzymes worked optimally at alkaline pHs, for keratinase at pH 8–12, and for disulfide reductase at pH 8–10. Optimum temperature for the extracellular keratinase was within 40–70 °C, while that for disulfide reductase was 35 °C. When the purified keratinase was mixed with purified disulfide reductase, enzyme activities on the natural keratin substrates (feather and wool) were greatly increased compared to activity of each enzyme alone, activity of proteinase K or activity of purified keratinase in the presence of reducing agents. The mutual action of the two enzymes on feather was examined by Scanning Electron Microscope.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 1, Issue 2, April 2012, Pages 152–158
نویسندگان
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