Enhanced production of amylase from Bacillus sp. using groundnut shell and cassava waste as a substrate under process optimization: Waste to wealth approach

The consumption of bi-substrates (groundnut shell and cassava waste) in order to produce an amylase enzyme by Bacillus sp. using solid stated fermentation was carried out by applying Box-Behnken design (BBD). Initial pH, incubation time, concentrations of groundnut shell (GNS) and cassava waste (CW) variables were generated for each fermentation conditions of the Bacillus sp. to optimize the amylase production. Maximum yield of amylase (866 U/mL) was achieved by Bacillus sp. at the favorable conditions of the fermentation; 60 h, pH 7.0, 2 g/L GNS and 2 g/L CW. It was revealed that the combination of bi-substrates might be used in industries to produce high amount of value added amylase.